%PDF-1.4 % 8 0 obj <> endobj 56 0 obj <>stream /Artifact <>BDC q 1 i 0 841.89 595.276 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n 0 0 0 0 k /GS1 gs 0 453.544 595.2 388.496 re f EMC /Figure <>BDC Q q 1 i -8.504 850.394 603.78 -396.85 re W* n 0 841.89 595.276 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS1 gs q 597.871 0 0 391.198 -1.3235 452.192 cm /Im1 Do Q EMC /P <>BDC Q q 1 i 0 841.89 595.276 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS2 gs BT /F5 1 Tf 7 0 0 7 42.5196 28.6398 Tm 0 0 0 1 k 0 Tc 0 Tw (20)Tj ET EMC /P <>BDC BT /F6 1 Tf 7 0 0 7 50.8077 28.6398 Tm ( )Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 42.5189 379.3701 Tm -.015 Tc (In seasonal-calving systems of milk production, early pregnancy)Tj ET EMC /P <>BDC BT 7.76 0 0 8 253.2265 379.3701 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 367.3701 Tm -.015 Tc (detection is a useful management tool to improve herd fertility)Tj ET EMC /P <>BDC BT 7.76 0 0 8 250.5183 367.3701 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 355.3702 Tm -.015 Tc [(per)-11.9(formance. Pregnancy-associated glycoproteins \(P)69.7(AG\) are proteins)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 269.7804 355.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 343.3702 Tm -.015 Tc (secreted by binucleate cells from the trophoblast of the developing)Tj ET EMC /P <>BDC BT 7.76 0 0 8 264.7192 343.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 331.3702 Tm -.015 Tc [(embr)-12(yo and are detectable in blood and milk four weeks after pregnancy)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 284.9879 331.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 319.3702 Tm -.015 Tc (establishment. The current gold standard for pregnancy diagnosis in)Tj ET EMC /P <>BDC BT 7.76 0 0 8 269.2742 319.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 307.3702 Tm -.015 Tc [(cattle is based on transrectal ultrasound to visualise a viable embr)-11.9(yo. )]TJ ET EMC /P <>BDC BT 7.76 0 0 8 270.8336 307.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 295.3702 Tm [(P)69.7(AG assays can be used as an alternative method to determine)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 256.697 295.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 283.3702 Tm [(pregnancy status and embr)-11.9(yo mortality)73.9(. The P)69.7(AG assay uses a plate)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 272.815 283.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 271.3702 Tm [(reader to measure absorbance in both samples \(S\) with unknown P)69.7(A)0(G)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 282.7784 271.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 259.3702 Tm [(concentrations and negative controls \(N\) with no P)69.7(AG present \(i.e., a)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 279.4183 259.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 247.3702 Tm (blank\). The S-N absorbance value is used to indicate the presence or)Tj ET EMC /P <>BDC BT 7.76 0 0 8 277.0804 247.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 235.3702 Tm [(absence of pregnancy)73.9(. Hereafter the P)69.7(AG S-N value is referred to)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 263.4258 235.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 223.3702 Tm [(simply as P)69.7(AG.)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 92.3323 223.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 211.3702 Tm -.015 Tc [(Pregnant dams have a well characterised P)69.7(AG profile in blood and milk,)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 279.054 211.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 199.3702 Tm -.015 Tc [(with an initial peak at weeks four to six in pregnancy)73.9(, followed by a)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 264.9606 199.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 187.3702 Tm -.015 Tc [(decline to nadir between weeks seven and 10, and a recover)-12(y thereafter)73.9(.)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 282.6608 187.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 175.3702 Tm -.015 Tc [(The objective of this study was to investigate factors that influence P)69.7(A)0(G)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 278.1383 175.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 163.3702 Tm -.015 Tc [(in seasonal-calving, pasture-based dair)-12(y cows. Spring-calving dair)-12(y cows)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 281.573 163.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 151.3702 Tm -.015 Tc [(\(n = 499\) located in three T)71.7(eagasc resear)17.8(ch farms were enrolled in the)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 274.1981 151.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 139.3702 Tm -.015 Tc [(study)73.9(. W)39.7(eekly milk samples were collected from each animal enrolled)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 271.3956 139.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 127.3702 Tm -.015 Tc [(from weeks five to 21 after artificial insemination \(AI\) to determine P)69.7(AG.)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 280.28 127.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 115.3702 Tm ( )Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 8.73 0 0 9 42.5189 103.3702 Tm .07 .94 .65 .31 k -.015 Tc [(Dam and sire c)17.8(har)23.9(acteristics)]TJ ET EMC /P <>BDC BT 8.73 0 0 9 161.8781 103.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC /GS2 gs BT /F3 1 Tf 7.76 0 0 8 42.5189 91.3702 Tm 0 0 0 1 k -.015 Tc [(There was a trend for declining milk P)69.7(AG with increasing parity number)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 279.4963 91.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 79.3702 Tm (\()Tj ET EMC /P <>BDC BT /F4 1 Tf 7.76 0 0 8 45.134 79.3702 Tm -.015 Tc (Figure 1)Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 73.6986 79.3702 Tm (\). An interaction between parity and week post insemination)Tj ET EMC /P <>BDC BT 7.76 0 0 8 274.4579 79.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5189 67.3702 Tm -.015 Tc [(was obser)-12(ved, whereby older cows \(parity 3+\) tended to have lesser P)69.7(A)0(G)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 283.7172 67.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 379.3701 Tm -.015 Tc (values at the initial peak and nadir compared to parity 1 and 2 cows.)Tj ET EMC /P <>BDC BT 7.76 0 0 8 536.7704 379.3701 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 367.3701 Tm -.015 Tc [(Cows with the greatest fertility sub-index had greater P)69.7(AG at weeks 5)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 538.4798 367.3701 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 355.3702 Tm -.015 Tc (and 6 post insemination compared with cows with the least or)Tj ET EMC /P <>BDC BT 7.76 0 0 8 516.5789 355.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 343.3702 Tm -.015 Tc (intermediate fertility sub-indexes, but differences were not detected)Tj ET EMC /P <>BDC BT 7.76 0 0 8 533.5035 343.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 331.3702 Tm -.015 Tc (thereafter \()Tj ET EMC /P <>BDC BT /F4 1 Tf 7.76 0 0 8 345.812 331.3702 Tm (Figure 2)Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 374.3766 331.3702 Tm [(\). Sires of the gestating embr)-11.9(yo with the greatest)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 536.8398 331.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 319.3702 Tm -.015 Tc [(daughter fertility sub-index had greater nadir P)69.7(AG than sires with)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 524.4574 319.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 307.3702 Tm -.015 Tc (intermediate and least fertility sub-index values \(1.46, 1.34 and 1.31,)Tj ET EMC /P <>BDC BT 7.76 0 0 8 537.8647 307.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 295.3702 Tm -.015 Tc [(respectively\), and reached nadir P)69.7(AG later \(weeks 10, 8 and 9,)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 514.672 295.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 283.3702 Tm -.015 Tc (respectively\). A significant effect of sire \()Tj ET EMC /P <>BDC BT /F4 1 Tf 7.76 0 0 8 441.3999 283.3702 Tm (Figure 3)Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 469.9645 283.3702 Tm [(\) was obser)-12(ved on initial)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 550.2725 283.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 271.3702 Tm -.015 Tc [(peak P)69.7(AG \(range 1.4-2.2\), nadir P)69.7(AG \(range 0.5-1.2\) and post-nadir)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 533.4766 271.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 259.3702 Tm -.015 Tc [(recover)-11.9(y peak P)69.7(AG \(range 2.0-3.0\). An interaction between milk yield)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 538.9758 259.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 247.3702 Tm -.015 Tc [(and week post insemination was obser)-11.9(ved, whereby P)69.7(AG decreased as)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 542.2584 247.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 235.3702 Tm -.015 Tc (milk yield increased.)Tj ET EMC /P <>BDC BT 7.76 0 0 8 375.8355 235.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 223.3702 Tm ( )Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 8.73 0 0 9 308.9752 211.3702 Tm .07 .94 .65 .31 k -.015 Tc [(Calf c)17.8(har)23.9(acteristics)]TJ ET EMC /P <>BDC BT 8.73 0 0 9 388.1416 211.3702 Tm 0 .68 .9 0 k 0 Tc ( )Tj ET EMC /P <>BDC /GS2 gs BT /F3 1 Tf 7.76 0 0 8 308.9752 199.3702 Tm 0 0 0 1 k -.015 Tc [(Pregnancies with female offspring had greater initial peak P)69.7(AG \(1.83\) and)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 552.308 199.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 187.3702 Tm -.015 Tc [(lesser P)69.7(AG values at post-nadir recover)-11.9(y \(2.50\) versus pregnancies with)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 543.3604 187.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 175.3702 Tm -.015 Tc (male offspring \(1.67 and 2.56, respectively\). Pregnancies that)Tj ET EMC /P <>BDC BT 7.76 0 0 8 513.0636 175.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 163.3702 Tm -.015 Tc (subsequently resulted in low birth weight calves \(< 30 kg\) had greater)Tj ET EMC /P <>BDC BT 7.76 0 0 8 541.0153 163.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 151.3702 Tm -.015 Tc [(initial peak P)69.7(AG \(1.84\) than pregnancies with medium \(30-38 kg = 1.73\))]TJ ET EMC /P <>BDC BT 7.76 0 0 8 550.174 151.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 139.3702 Tm -.015 Tc (and large \(> 38 kg = 1.69\) birth weight calves \()Tj ET EMC /P <>BDC BT /F4 1 Tf 7.76 0 0 8 465.8516 139.3702 Tm (Figure 4)Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 494.4163 139.3702 Tm (\).)Tj ET EMC /P <>BDC BT 7.76 0 0 8 499.1421 139.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 127.3702 Tm ( )Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 8.73 0 0 9 308.9752 115.3702 Tm .07 .94 .65 .31 k -.015 Tc (Conclusion)Tj ET EMC /P <>BDC BT 8.73 0 0 9 355.8641 115.3702 Tm 0 .68 .9 0 k 0 Tc ( )Tj ET EMC /P <>BDC /GS2 gs BT /F3 1 Tf 7.76 0 0 8 308.9752 103.3702 Tm 0 0 0 1 k -.015 Tc [(The overall temporal pattern of variation in milk P)69.7(AG values in seasonal-)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 91.3702 Tm (calving pasture-based cows is consistent with previous reports from high-)Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 79.3702 Tm (yielding cows fed a total mixed ration. The majority of the variation)Tj ET EMC /P <>BDC BT 7.76 0 0 8 532.1921 79.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 308.9752 67.3702 Tm -.015 Tc [(obser)-11.9(ved in milk P)69.7(AG profiles occurred before week 12 post)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 506.508 67.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT /F6 1 Tf 7 0 0 7 68.0315 28.6398 Tm (TRESEARCH)Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 7 0 0 7 109.1284 28.6398 Tm .78 .02 .98 .09 k ( | )Tj ET EMC /P <>BDC /GS2 gs BT /F6 1 Tf 7 0 0 7 114.8265 28.6398 Tm 0 0 0 1 k (SUMMER 2020)Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 7 0 0 7 162.9305 28.6398 Tm .78 .02 .98 .09 k ( | )Tj ET EMC /P <>BDC /GS2 gs BT /F6 1 Tf 7 0 0 7 168.6285 28.6398 Tm 0 0 0 1 k [(V)36.9(OLUME 15 : NUMBER 2)]TJ ET EMC /P <>BDC /GS1 gs BT 16 0 0 16 42.5294 784.3701 Tm 0 0 0 0 k -.015 Tc [(A)36.8(GRI)]TJ ET EMC /P <>BDC BT /F7 1 Tf 20 0 0 20 79.7967 784.3701 Tm .06 .07 .09 .15 k 0 Tc ( )Tj ET EMC /Artifact <>BDC .07 .94 .65 .31 k 42.52 777.161 42.519 1.418 re f EMC /Artifact <>BDC 0 448.205 595.2 5.669 re f EMC /Artifact <>BDC .06 .07 .09 .15 K 0 J 0 j .5 w 10 M [] 0 d q 1 0 0 1 297.638 382.527 cm 0 0 m 0 -314.496 l S Q EMC /P <>BDC BT /F1 1 Tf 35 0 0 35 42.5196 525.3405 Tm .255 .929 .827 .141 k [(F)44.9(actors affecting )]TJ ET EMC /P <>BDC BT 35 0 0 35 335.9624 525.3405 Tm ( )Tj ET EMC /P <>BDC BT 35 0 0 35 42.5196 482.5426 Tm [(pr)12(e)17.8(gnanc)23.9(y pr)12(oteins)]TJ ET EMC /P <>BDC BT 12.87 0 0 13 42.5161 422.9058 Tm [(TEA)36.8(GASC )]TJ ET EMC /P <>BDC /GS2 gs BT 12.87 0 0 13 106.4184 422.9058 Tm 0 0 0 1 k [(r)11.9(esear)17.8(c)17.8(hers in)29.7(v)11.9(estig)-5.8(ated factors influencing pr)11.9(e)17.8(gnanc)23.9(y-associated)]TJ ET EMC /P <>BDC BT 12.87 0 0 13 523.2787 422.9058 Tm ( )Tj ET EMC /P <>BDC BT 12.87 0 0 13 42.5161 405.9059 Tm [(gl)11.9(ycopr)11.9(oteins in seasonal calving,)36.8( pastur)11.9(e-based dair)11.9(y co)23.9(ws.)36.8( )]TJ ET EMC Q endstream endobj 57 0 obj <>/XObject<>/ExtGState<>>> endobj 110 0 obj <> endobj 114 0 obj <> endobj 118 0 obj <> endobj 142 0 obj <> endobj 156 0 obj <> endobj 331 0 obj <>stream /P <>BDC q 1 i 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS2 gs BT /F6 1 Tf 7 0 0 7 542.5214 28.6398 Tm 0 0 0 1 k 0 Tc 0 Tw ( )Tj ET EMC /P <>BDC BT /F5 1 Tf 7 0 0 7 544.4675 28.6398 Tm (21)Tj ET EMC /P <>BDC BT /F3 1 Tf 7.76 0 0 8 42.5177 283.3702 Tm -.015 Tc [(insemination. Our findings indicate that calf sex, calf weight, parity)73.9(, dam)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 283.0947 283.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 271.3702 Tm -.015 Tc (and sire fertility sub-index, milk yield, and sire are all associated with milk)Tj ET EMC /P <>BDC BT 7.76 0 0 8 283.8619 271.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 259.3702 Tm -.015 Tc [(P)69.7(AG values obser)-11.9(ved from weeks five to 21 post AI. The use of a milk)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 269.8335 259.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 247.3702 Tm -.015 Tc (sample to diagnose pregnancy is a convenient method for pregnancy)Tj ET EMC /P <>BDC BT 7.76 0 0 8 273.4402 247.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 235.3702 Tm -.015 Tc (diagnosis, especially for farmers participating in milk recording, due to)Tj ET EMC /P <>BDC BT 7.76 0 0 8 275.2483 235.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 223.3702 Tm -.015 Tc (the low technical inputs required. The ability to determine pregnancy)Tj ET EMC /P <>BDC BT 7.76 0 0 8 272.928 223.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 211.3702 Tm -.015 Tc [(status through milk P)69.7(AG may be useful to improve reproductive)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 253.6077 211.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 199.3702 Tm -.015 Tc [(management in dair)-11.9(y herds.)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 136.4523 199.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 8 0 0 8 42.5193 187.3702 Tm ( )Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 9 0 0 9 42.5193 175.3702 Tm .07 .94 .65 .31 k [(Ac)17.8(kno)23.9(wledgements)]TJ ET EMC /P <>BDC BT 9 0 0 9 130.0016 175.3702 Tm 0 .68 .9 0 k ( )Tj ET EMC /P <>BDC /GS2 gs BT /F3 1 Tf 8 0 0 8 42.5193 163.3702 Tm 0 0 0 1 k [(This resear)17.8(ch is funded by the T)71.8(eagasc W)39.8(alsh Scholarships)]TJ ET EMC /P <>BDC BT 8 0 0 8 246.62 163.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 42.5193 151.3702 Tm [(Programme, and from the T)71.8(eagasc Dair)-12(y Levy T)75.9(rust and Irish)]TJ ET EMC /P <>BDC BT 8 0 0 8 256.0412 151.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 42.5193 139.3702 Tm (Department of Agriculture, Food and the Marine RSF 13S528. )Tj ET EMC /P <>BDC BT 8 0 0 8 263.6073 139.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 42.5193 127.3702 Tm (The support of IDEXX USA is also acknowledged.)Tj ET EMC /P <>BDC BT 8 0 0 8 215.3353 127.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 42.5193 115.3702 Tm ( )Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 9 0 0 9 42.5193 103.3702 Tm .07 .94 .65 .31 k [(A)29.8(uthors)]TJ ET EMC /P <>BDC BT 9 0 0 9 78.2603 103.3702 Tm 0 .68 .9 0 k ( )Tj ET EMC /P <>BDC /GS2 gs BT /F4 1 Tf 8 0 0 8 42.5193 91.3702 Tm 0 0 0 1 k (Rachel Doyle)Tj ET EMC /P <>BDC BT /F3 1 Tf 8 0 0 8 90.8473 91.3702 Tm ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 79.3702 Tm -.015 Tc [(T)71.7(eagasc W)39.7(alsh Scholar)49.7(, University of Missouri and T)71.7(eagasc Animal &)]TJ ET EMC /P <>BDC BT 7.76 0 0 8 267.6912 79.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 7.76 0 0 8 42.5177 67.3702 Tm -.015 Tc [(Grassland Resear)17.8(ch and Innovation Centre, Moorepark, Fermoy)73.9(, Co. Cork)]TJ ET EMC /P <>BDC BT 8 0 0 8 285.845 67.3702 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT /F4 1 Tf 8 0 0 8 308.976 283.3702 Tm [(Mar)-12(y Herlihy)]TJ ET EMC /P <>BDC BT /F3 1 Tf 8 0 0 8 357.6237 283.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 271.3702 Tm [(T)71.8(eagasc Animal & Grassland Resear)17.8(ch and Innovation Centre,)]TJ ET EMC /P <>BDC BT 8 0 0 8 526.3152 271.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 259.3702 Tm [(Moorepark, Fermoy)74(, Co. Cork )]TJ ET EMC /P <>BDC BT 8 0 0 8 416.3602 259.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 247.3702 Tm ( )Tj ET EMC /P <>BDC BT /F4 1 Tf 8 0 0 8 308.976 235.3702 Tm (Matthew Lucy)Tj ET EMC /P <>BDC BT /F3 1 Tf 8 0 0 8 362.192 235.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 223.3702 Tm (Professor of Animal Science, University of Missouri, Columbia,)Tj ET EMC /P <>BDC BT 8 0 0 8 526.9439 223.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 211.3702 Tm (Missouri, USA)Tj ET EMC /P <>BDC BT 8 0 0 8 357.536 211.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 199.3702 Tm ( )Tj ET EMC /P <>BDC BT /F4 1 Tf 8 0 0 8 308.976 187.3702 Tm (Stephen Butler)Tj ET EMC /P <>BDC BT /F3 1 Tf 8 0 0 8 365.048 187.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 175.3702 Tm [(Principal Resear)17.8(ch Officer)49.8(, T)71.8(eagasc Animal & Grassland Resear)17.8(ch and)]TJ ET EMC /P <>BDC BT 8 0 0 8 551.1262 175.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 163.3702 Tm [(Innovation Centre, Moorepark, Fermoy)74(, Co. Cork)]TJ ET EMC /P <>BDC BT 8 0 0 8 482.6721 163.3702 Tm ( )Tj ET EMC /P <>BDC BT 8 0 0 8 308.976 151.3702 Tm (Correspondence: stephen.butler@teagasc.ie )Tj ET EMC /P <>BDC BT 8 0 0 8 466.5039 151.3702 Tm ( )Tj ET EMC /P <>BDC BT /F6 1 Tf 7 0 0 7 344.877 28.6398 Tm (TRESEARCH)Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 7 0 0 7 385.9739 28.6398 Tm .78 .02 .98 .09 k ( | )Tj ET EMC /P <>BDC /GS2 gs BT /F6 1 Tf 7 0 0 7 391.6719 28.6398 Tm 0 0 0 1 k (SUMMER 2020)Tj ET EMC /P <>BDC /GS1 gs BT /F5 1 Tf 7 0 0 7 439.776 28.6398 Tm .78 .02 .98 .09 k ( | )Tj ET EMC /P <>BDC /GS2 gs BT /F6 1 Tf 7 0 0 7 445.474 28.6398 Tm 0 0 0 1 k [(V)36.9(OLUME 15 : NUMBER 2)]TJ ET EMC /Artifact <>BDC 0 0 0 0 k /GS1 gs 308.976 68.032 51.024 62.362 re f EMC /Figure <>BDC Q q 1 i 308.976 130.394 51.024 -62.362 re W* n 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS1 gs q 51.842 .48845 -.57288 60.802 310.368 67.495 cm /Im3 Do Q EMC /Artifact <>BDC Q q 1 i 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n .007 .094 .065 .031 k /GS1 gs 365.257 68.032 51.024 62.362 re f EMC /Figure <>BDC Q q 1 i 365.257 130.394 51.024 -62.362 re W* n 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS1 gs q 56.709 1.6189 -1.8737 65.632 363.399 64.654 cm /Im5 Do Q EMC /Artifact <>BDC Q q 1 i 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n 0 0 0 0 k /GS1 gs 421.539 68.032 51.023 62.362 re f EMC /Figure <>BDC Q q 1 i 421.539 130.394 51.023 -62.362 re W* n 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS1 gs q 54.238 0 0 65.247 420.12 66.515 cm /Im7 Do Q EMC /Artifact <>BDC Q q 1 i 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n .06 .07 .09 .15 K 0 J 0 j .5 w 10 M [] 0 d /GS1 gs q 1 0 0 1 297.589 286.911 cm 0 0 m 0 -208.858 l S Q EMC /P <>BDC BT /F6 1 Tf 16 0 0 16 515.2747 787.0821 Tm .06 .07 .09 .15 k -.015 Tc 0 Tw [(A)36.8(GRI)]TJ ET EMC /Artifact <>BDC .255 .929 .827 .141 k 510.244 777.161 42.519 1.418 re f EMC /Artifact <>BDC 0 0 0 0 k 42.519 544.355 243.78 36.421 re f EMC /P <>BDC BT /F2 1 Tf 7 0 0 7 42.5193 575.3994 Tm .78 .761 .741 .855 k 0 Tc [(FIGURE 1: Pr)17.8(egnanc)17.8(y-associated glycopr)17.8(otein pr)17.8(ofiles fr)17.8(om fiv)5.9(e to 21 weeks)]TJ ET EMC /P <>BDC BT 7 0 0 7 276.7813 575.3994 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 42.5193 564.3994 Tm [(post )36.9(AI f)17.8(or parity 1 \( )]TJ ET EMC /P <>BDC BT 7 0 0 7 108.0064 564.3994 Tm 1 0 0 0 k ( )Tj ET EMC /P <>BDC BT 7 0 0 7 111.8984 564.3994 Tm .78 .761 .741 .855 k [(\),)36.9( 2 \()]TJ ET EMC /P <>BDC BT 7 0 0 7 126.0323 564.3994 Tm 0 .68 .9 0 k -.05 Tc ( )Tj ET EMC /P <>BDC BT 7 0 0 7 132.4165 564.3994 Tm .78 .761 .741 .855 k 0 Tc [(\),)36.9( and 3+ \( \) lactating dair)17.8(y cows)17.8(.)]TJ ET EMC /P <>BDC BT /F8 1 Tf 7 0 0 7 239.4568 564.3994 Tm ( )Tj ET EMC /Artifact <>BDC 0 0 0 0 k 308.984 541.381 243.779 39.395 re f EMC /P <>BDC BT /F2 1 Tf 7 0 0 7 308.9838 575.3994 Tm .78 .761 .741 .855 k [(FIGURE 2: )36.9(W)54.9(eekly pr)17.8(egnanc)17.8(y-associated glycopr)17.8(otein pr)17.8(ofiles in milk fr)17.8(om fiv)5.9(e)]TJ ET EMC /P <>BDC BT 7 0 0 7 552.4524 575.3994 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 308.9838 564.3994 Tm [(to 21 weeks post )36.9(AI f)17.8(or cows with differ)17.8(ent dam fertility sub-index categories:)]TJ ET EMC /P <>BDC BT 7 0 0 7 550.7952 564.3994 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 308.9838 553.3994 Tm (least \(< )Tj /F9 1 Tf 7 0 1.4879 7 334.1907 553.3994 Tm ()Tj /F2 1 Tf 7 0 0 7 339.3997 553.3994 Tm ( 85\); intermediate \()Tj /F9 1 Tf 7 0 1.4879 7 398.9138 553.3994 Tm ()Tj /F2 1 Tf 7 0 0 7 404.1227 553.3994 Tm ( 85-)Tj /F9 1 Tf 7 0 1.4879 7 416.4427 553.3994 Tm ()Tj /F2 1 Tf 7 0 0 7 421.6516 553.3994 Tm [( 120\); and,)36.9( gr)17.8(eatest \(> )]TJ /F9 1 Tf 7 0 1.4879 7 492.3819 553.3994 Tm ()Tj /F2 1 Tf 7 0 0 7 497.5909 553.3994 Tm ( 120\).)Tj ET EMC /Artifact <>BDC 0 0 0 0 k 42.965 315.626 243.78 36.421 re f EMC /P <>BDC BT 7 0 0 7 42.9653 346.6709 Tm .78 .761 .741 .855 k [(FIGURE 3: )36.9(V)91.8(ariation between sir)17.8(es \(n = 19\) of the dev)5.9(eloping f)17.8(oetus on )]TJ ET EMC /P <>BDC BT 7 0 0 7 263.023 346.6709 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 42.9653 335.6709 Tm [(mean v)29.8(alues f)17.8(or initial peak P)74(A)36.9(G)23.9(,)36.9( nadir P)74(A)36.9(G and post-nadir r)17.8(ecov)5.9(er)17.8(y peak)]TJ ET EMC /P <>BDC BT 7 0 0 7 272.7362 335.6709 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 42.9653 324.671 Tm [(P)74(A)36.9(G in milk.)]TJ ET EMC /Artifact <>BDC 0 0 0 0 k 308.989 311.608 243.779 40.087 re f EMC /P <>BDC BT 7 0 0 7 308.989 346.3188 Tm .78 .761 .741 .855 k [(FIGURE 4: )36.9(W)54.9(eekly pr)17.8(egnanc)17.8(y-associated glycopr)17.8(otein pr)17.8(ofiles in milk fr)17.8(om)]TJ ET EMC /P <>BDC BT 7 0 0 7 539.1426 346.3188 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 308.989 335.3188 Tm [(cows with male or female calv)5.9(es \(lines\),)36.9( or those with low \(< 30 kg\),)36.9( medium)]TJ ET EMC /P <>BDC BT 7 0 0 7 547.8108 335.3188 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 308.989 324.3188 Tm [(\(30-38 kg\) or high \(> 38 kg\) birth weight \(solid bar)12(s\).)36.9( )]TJ ET EMC /Artifact <>BDC .007 .094 .065 .031 k 478.539 68.032 51.023 62.362 re f EMC /Figure <>BDC Q q 1 i 478.539 130.394 51.023 -62.362 re W* n 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n /GS1 gs q 54.347 0 0 65.47 476.903 66.494 cm /Im9 Do Q EMC /Artifact <>BDC Q q 1 i 0 841.89 595.275 -841.89 re 297.6 421.08 m W n 0 842.04 595.2 -841.92 re W n .78 .02 .98 .09 K 0 J 0 j 1 w 10 M [] 0 d /GS1 gs q 1 0 0 1 343.066 516.465 cm 0 0 m 0 -120.368 l S Q EMC q 1 0 0 -1 -1191.276 878.88977 cm /Fm1 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm2 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm3 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm4 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm5 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm6 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm7 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm8 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm9 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm10 Do Q /Artifact <>BDC .043 .133 .827 0 k 345.724 395.406 2.5511 90.341 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 348.275 396.096 2.5509 106.844 re f EMC /Artifact <>BDC 0 .68 .9 0 k 350.826 396.096 2.552 102.317 re f EMC /Artifact <>BDC .043 .133 .827 0 k 357.897 395.39 2.5511 84.722 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 360.275 396.096 2.5509 94.298 re f EMC /Artifact <>BDC 0 .68 .9 0 k 362.826 396.096 2.552 102.317 re f EMC /Artifact <>BDC .043 .133 .827 0 k 370.795 395.877 2.5509 76.871 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 373.275 396.096 2.551 73.591 re f EMC /Artifact <>BDC 0 .68 .9 0 k 375.826 396.096 2.552 71.913 re f EMC /Artifact <>BDC .043 .133 .827 0 k 382.149 395.39 2.552 76.026 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 384.639 396.097 2.5509 61.59 re f EMC /Artifact <>BDC 0 .68 .9 0 k 387.19 396.096 2.5509 63.428 re f EMC /Artifact <>BDC .043 .133 .827 0 k 394.15 395.872 2.5511 75.552 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 396.639 396.097 2.5509 62.681 re f EMC /Artifact <>BDC 0 .68 .9 0 k 399.19 396.096 2.5509 61.59 re f EMC /Artifact <>BDC .043 .133 .827 0 k 407.177 395.887 2.5511 79.618 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 409.639 396.097 2.551 63.427 re f EMC /Artifact <>BDC 0 .68 .9 0 k 412.19 396.096 2.5511 64.649 re f EMC /Artifact <>BDC .043 .133 .827 0 k 419.192 395.897 2.551 81.842 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 421.639 396.096 2.551 76.652 re f EMC /Artifact <>BDC 0 .68 .9 0 k 424.19 396.096 2.551 73.59 re f EMC /Artifact <>BDC .043 .133 .827 0 k 431.031 395.39 2.551 89.275 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 433.639 396.096 2.551 84.016 re f EMC /Artifact <>BDC 0 .68 .9 0 k 436.19 396.096 2.551 85.708 re f EMC /Artifact <>BDC .043 .133 .827 0 k 443.309 395.895 2.5511 88.856 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 445.639 396.096 2.551 88.569 re f EMC /Artifact <>BDC 0 .68 .9 0 k 448.19 396.096 2.551 88.568 re f EMC /Artifact <>BDC .043 .133 .827 0 k 455.301 395.895 2.5521 88.932 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 457.639 396.096 2.551 92.344 re f EMC /Artifact <>BDC 0 .68 .9 0 k 460.19 396.097 2.551 96.368 re f EMC /Artifact <>BDC .043 .133 .827 0 k 467.301 395.862 2.5511 90.804 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 469.639 396.096 2.551 92.344 re f EMC /Artifact <>BDC 0 .68 .9 0 k 472.19 396.097 2.5511 96.368 re f EMC /Artifact <>BDC .043 .133 .827 0 k 479.301 395.882 2.5511 92.868 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 481.639 396.096 2.551 92.344 re f EMC /Artifact <>BDC 0 .68 .9 0 k 484.19 396.097 2.5511 98.25 re f EMC /Artifact <>BDC .043 .133 .827 0 k 492.3 395.862 2.5511 90.98 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 494.639 396.096 2.5509 99.967 re f EMC /Artifact <>BDC 0 .68 .9 0 k 497.19 396.097 2.5511 104.956 re f EMC /Artifact <>BDC .043 .133 .827 0 k 504.3 395.862 2.5511 93.055 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 506.639 396.096 2.5509 98.251 re f EMC /Artifact <>BDC 0 .68 .9 0 k 509.19 396.096 2.5511 104.016 re f EMC /Artifact <>BDC .043 .133 .827 0 k 516.281 395.406 2.551 93.597 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 518.639 396.096 2.5509 104.957 re f EMC /Artifact <>BDC 0 .68 .9 0 k 521.19 396.096 2.5511 106.486 re f EMC /Artifact <>BDC .043 .133 .827 0 k 528.286 395.867 2.5511 102.16 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 530.639 396.096 2.5509 106.223 re f EMC /Artifact <>BDC 0 .68 .9 0 k 533.19 396.096 2.5511 111.192 re f EMC /Artifact <>BDC .043 .133 .827 0 k 540.252 395.862 2.551 93.743 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 542.639 396.096 2.5509 106.223 re f EMC /Artifact <>BDC 0 .68 .9 0 k 545.19 396.096 2.5511 111.192 re f EMC /Artifact <>BDC .21 .7 .92 .7 k q 1 0 0 1 350.77 499.92 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.8191 -1.33 -4.8191 0 c -4.8191 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 363.73 488.88 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.8191 -1.33 -4.8191 0 c -4.8191 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 375.73 476.4 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.8191 -1.33 -4.8191 0 c -4.8191 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 387.369 465.84 cm 0 0 m 0 -1.33 -1.0793 -2.4095 -2.409 -2.4095 c -3.7388 -2.4095 -4.818 -1.33 -4.818 0 c -4.818 1.33 -3.7388 2.4095 -2.409 2.4095 c -1.0793 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 399.969 463.2 cm 0 0 m 0 -1.33 -1.0794 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0794 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 411.49 467.28 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 424.69 474.24 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 436.69 484.32 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 448.69 486.24 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 460.69 487.2 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 473.65 487.2 cm 0 0 m 0 -1.33 -1.0794 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0794 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 485.65 489.36 cm 0 0 m 0 -1.33 -1.0794 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0794 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 497.65 492.24 cm 0 0 m 0 -1.33 -1.0794 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0794 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 509.65 491.28 cm 0 0 m 0 -1.33 -1.0794 -2.4095 -2.4095 -2.4095 c -3.7395 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7395 2.4095 -2.4095 2.4095 c -1.0794 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 521.65 498.24 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 533.65 501.36 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Artifact <>BDC q 1 0 0 1 545.65 501.36 cm 0 0 m 0 -1.33 -1.0795 -2.4095 -2.4095 -2.4095 c -3.7396 -2.4095 -4.819 -1.33 -4.819 0 c -4.819 1.33 -3.7396 2.4095 -2.4095 2.4095 c -1.0795 2.4095 0 1.33 0 0 c f* Q EMC /Figure <>BDC /GS4 gs q 210.72 0 0 60.96 343.464 449.903 cm /Im11 Do Q EMC /Artifact <>BDC 1 0 0 0 k /GS1 gs q 1 0 0 1 349.409 495.213 cm 0 0 m 2.4089 -4.819 l -2.4091 -4.819 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 361.692 484.664 cm 0 0 m 2.4101 -4.818 l -2.409 -4.819 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 373.509 467.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 385.509 462.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 410.509 466.277 cm 0 0 m 2.4099 -4.818 l -2.409 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 434.509 484.277 cm 0 0 m 2.4099 -4.818 l -2.409 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 458.509 494.277 cm 0 0 m 2.4099 -4.818 l -2.409 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 471.509 494.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 483.509 497.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 495.509 501.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 544.509 507.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC .21 .7 .92 .7 K q 1 0 0 1 348.955 500.315 cm 0 0 m 12.627 -11.425 l 24.307 -24.094 l 35.846 -34.656 l 48.615 -37.271 l 60.154 -33.271 l 74.307 -26.194 l 85.384 -15.733 l 97.231 -14.656 l 109.231 -13.425 l 122.154 -13.579 l 133.846 -12.04 l 146.307 -8.81 l 158.611 -10.04 l 170.154 -2.348 l 182.461 .8831 l 194.154 .421 l S Q EMC /Artifact <>BDC q 1 0 0 1 398.509 465.277 cm 0 0 m 2.4099 -4.818 l -2.409 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 422.509 477.277 cm 0 0 m 2.4099 -4.818 l -2.409 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 447.509 489.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 507.509 496.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 519.509 500.277 cm 0 0 m 2.41 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 532.509 505.277 cm 0 0 m 2.4099 -4.818 l -2.4091 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC 1 0 0 0 K q 1 0 0 1 348.955 491.811 cm 0 0 m 13.077 -10.613 l 24.769 -28.152 l 37.384 -32.459 l 49.846 -29.763 l 61.692 -29.075 l 74 -17.383 l 85.692 -10.459 l 98.307 -5.669 l 110 -.306 l 122.461 -.613 l 134.615 2.156 l 146.307 6.156 l 158.769 1.694 l 170.615 5.233 l 183.384 10.31 l 195.462 12.156 l S Q EMC /P <>BDC /GS2 gs BT /F3 1 Tf 7 0 0 7 322.7239 515.5583 Tm 0 0 0 1 k 0 Tc 0 Tw (2.50)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 515.5583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 503.3582 Tm (2.25)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 503.3582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 491.1582 Tm (2.00)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 491.1582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 478.9583 Tm (1.75)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 478.9583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 466.7583 Tm (1.50)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 466.7583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 454.5583 Tm (1.25)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 454.5583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 442.3582 Tm (1.00)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 442.3582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 430.1583 Tm (0.75)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 430.1583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 417.9583 Tm (0.50)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 417.9583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 405.7583 Tm (0.25)Tj ET EMC /P <>BDC BT 7 0 0 7 336.7869 405.7583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 322.7239 393.5583 Tm (0.00)Tj ET EMC /P <>BDC BT /F4 1 Tf 0 8 -8 0 314.9003 440.7014 Tm [(P)69.8(AG S-N)]TJ ET EMC /P <>BDC BT 8 0 0 8 418.9749 370.0693 Tm [(W)39.8(eeks post AI)]TJ ET EMC /P <>BDC BT /F3 1 Tf 7 0 0 7 343.8796 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 347.8977 384.8033 Tm (5)Tj ET EMC /P <>BDC BT 7 0 0 7 353.784 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 359.811 384.8033 Tm (6)Tj ET EMC /P <>BDC BT 7 0 0 7 365.784 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 371.811 384.8033 Tm (7)Tj ET EMC /P <>BDC BT 7 0 0 7 377.784 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 383.811 384.8033 Tm (8)Tj ET EMC /P <>BDC BT 7 0 0 7 388.0506 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 396.0866 384.8033 Tm (9)Tj ET EMC /P <>BDC BT 7 0 0 7 400.8065 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 406.8335 384.8033 Tm (10)Tj ET EMC /P <>BDC BT 7 0 0 7 416.7931 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 418.802 384.8033 Tm (11)Tj ET EMC /P <>BDC BT 7 0 0 7 427.1173 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 431.1354 384.8033 Tm (12)Tj ET EMC /P <>BDC BT 7 0 0 7 439.4507 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 443.4687 384.8033 Tm (13)Tj ET EMC /P <>BDC BT 7 0 0 7 452.7931 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 454.802 384.8033 Tm (14)Tj ET EMC /P <>BDC BT 7 0 0 7 463.4507 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 467.4687 384.8033 Tm (15)Tj ET EMC /P <>BDC BT 7 0 0 7 477.1081 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 479.1169 384.8033 Tm (16)Tj ET EMC /P <>BDC BT 7 0 0 7 488.4506 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 492.4687 384.8033 Tm (17)Tj ET EMC /P <>BDC BT 7 0 0 7 500.6107 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 504.6287 384.8033 Tm (18)Tj ET EMC /P <>BDC BT 7 0 0 7 514.1263 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 516.1354 384.8033 Tm (19)Tj ET EMC /P <>BDC BT 7 0 0 7 524.4506 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 528.4688 384.8033 Tm (20)Tj ET EMC /P <>BDC BT 7 0 0 7 538.0529 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 540.0619 384.8033 Tm (21)Tj ET EMC /Artifact <>BDC .78 .02 .98 .09 K /GS1 gs q 1 0 0 1 342.723 395.906 cm 0 0 m 207.031 0 l S Q EMC /P <>BDC /GS2 gs BT 7 0 0 7 72.0123 515.5583 Tm (3.5)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 515.5583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 78.0393 498.3582 Tm (3)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 498.3582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 72.0123 481.1582 Tm (2.5)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 481.1582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 78.0393 463.9583 Tm (2)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 463.9583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 72.0123 446.7583 Tm (1.5)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 446.7583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 78.0393 429.5583 Tm (1)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 429.5583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 72.0123 412.3582 Tm (0.5)Tj ET EMC /P <>BDC BT 7 0 0 7 82.0574 412.3582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 78.0393 395.1583 Tm (0)Tj ET EMC /P <>BDC BT /F4 1 Tf 0 8 -8 0 62.9003 440.7014 Tm [(P)69.8(AG S-N)]TJ ET EMC /Artifact <>BDC /GS1 gs q 1 0 0 1 88.222 516.715 cm 0 0 m 0 -120.618 l S Q EMC q 1 0 0 -1 -1191.276 878.88977 cm /Fm11 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm12 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm13 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm14 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm15 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm16 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm17 Do Q /Artifact <>BDC q 1 0 0 1 87.73 395.906 cm 0 0 m 172.558 0 l S Q EMC /P <>BDC /GS2 gs BT 8 0 0 8 167.3828 373.0693 Tm (Bull)Tj ET EMC /P <>BDC BT /F3 1 Tf 6.79 0 0 7 85.4401 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 90.9809 384.8033 Tm 0 Tc (1)Tj ET EMC /P <>BDC BT 6.79 0 0 7 96.6011 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 100.2948 384.8033 Tm 0 Tc (2)Tj ET EMC /P <>BDC BT 6.79 0 0 7 105.7315 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 109.4252 384.8033 Tm 0 Tc (3)Tj ET EMC /P <>BDC BT 6.79 0 0 7 114.4426 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 118.1364 384.8033 Tm 0 Tc (4)Tj ET EMC /P <>BDC BT 6.79 0 0 7 123.7759 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 127.4697 384.8033 Tm 0 Tc (5)Tj ET EMC /P <>BDC BT 6.79 0 0 7 132.8426 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 136.5363 384.8033 Tm 0 Tc (6)Tj ET EMC /P <>BDC BT 6.79 0 0 7 142.1759 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 145.8696 384.8033 Tm 0 Tc (7)Tj ET EMC /P <>BDC BT 6.79 0 0 7 150.9758 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 154.6697 384.8033 Tm 0 Tc (8)Tj ET EMC /P <>BDC BT 6.79 0 0 7 160.0425 384.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 163.7363 384.8033 Tm 0 Tc (9)Tj ET EMC /P <>BDC BT 6.79 0 0 7 168.8336 384.8033 Tm ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 170.6804 384.8033 Tm -.015 Tc (10)Tj ET EMC /P <>BDC BT 6.79 0 0 7 179.9718 384.8033 Tm (11)Tj ET EMC /P <>BDC BT 6.79 0 0 7 188.7717 384.8033 Tm (12)Tj ET EMC /P <>BDC BT 6.79 0 0 7 198.1051 384.8033 Tm (13)Tj ET EMC /P <>BDC BT 6.79 0 0 7 207.5307 384.8033 Tm (14)Tj ET EMC /P <>BDC BT 6.79 0 0 7 216.5051 384.8033 Tm (15)Tj ET EMC /P <>BDC BT 6.79 0 0 7 225.3051 384.8033 Tm (16)Tj ET EMC /P <>BDC BT 6.79 0 0 7 234.6384 384.8033 Tm (17)Tj ET EMC /P <>BDC BT 6.79 0 0 7 242.9638 384.8033 Tm (18)Tj ET EMC /P <>BDC BT 6.79 0 0 7 250.6581 384.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 252.5051 384.8033 Tm -.015 Tc (19)Tj ET EMC /Artifact <>BDC .043 .133 .827 0 k /GS1 gs 90.141 395.406 1.7011 23.5 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 91.842 396.097 1.7009 62.382 re f EMC /Artifact <>BDC 0 .68 .9 0 k 93.261 396.096 1.701 84.505 re f EMC /Artifact <>BDC .043 .133 .827 0 k 99.496 395.406 1.7 29.557 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 101.196 396.097 1.701 60.257 re f EMC /Artifact <>BDC 0 .68 .9 0 k 102.615 396.096 1.701 102.105 re f EMC /Artifact <>BDC .043 .133 .827 0 k 108.496 395.406 1.7 22.014 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 110.196 396.097 1.701 63.114 re f EMC /Artifact <>BDC 0 .68 .9 0 k 111.615 396.096 1.701 70.562 re f EMC /Artifact <>BDC .043 .133 .827 0 k 117.496 395.406 1.7 29.557 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 119.196 396.097 1.701 57.057 re f EMC /Artifact <>BDC 0 .68 .9 0 k 120.615 396.096 1.701 75.476 re f EMC /Artifact <>BDC .043 .133 .827 0 k 126.496 395.406 1.7 24.414 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 128.196 396.097 1.701 64.029 re f EMC /Artifact <>BDC 0 .68 .9 0 k 129.615 396.096 1.701 82.219 re f EMC /Artifact <>BDC .043 .133 .827 0 k 135.496 395.406 1.7 35.271 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 137.196 396.097 1.701 74.314 re f EMC /Artifact <>BDC 0 .68 .9 0 k 138.615 396.096 1.701 84.505 re f EMC /Artifact <>BDC .043 .133 .827 0 k 144.496 395.406 1.7 28.528 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 146.196 396.097 1.701 60.861 re f EMC /Artifact <>BDC 0 .68 .9 0 k 147.615 396.096 1.701 83.696 re f EMC /Artifact <>BDC .043 .133 .827 0 k 153.496 395.406 1.7 27.728 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 155.196 396.097 1.701 65.661 re f EMC /Artifact <>BDC 0 .68 .9 0 k 156.615 396.096 1.701 91.095 re f EMC /Artifact <>BDC .043 .133 .827 0 k 162.496 395.406 1.7 20.681 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 164.196 396.097 1.701 58.042 re f EMC /Artifact <>BDC 0 .68 .9 0 k 165.615 396.096 1.701 91.095 re f EMC /Artifact <>BDC .043 .133 .827 0 k 171.496 395.406 1.7 29.557 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 173.196 396.097 1.701 61.661 re f EMC /Artifact <>BDC 0 .68 .9 0 k 174.615 396.097 1.701 74.904 re f EMC /Artifact <>BDC .043 .133 .827 0 k 180.496 395.406 1.7 26.985 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 182.196 396.097 1.701 71.946 re f EMC /Artifact <>BDC 0 .68 .9 0 k 183.615 396.097 1.701 77.761 re f EMC /Artifact <>BDC .043 .133 .827 0 k 189.496 395.406 1.7 21.353 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 191.196 396.097 1.701 64.029 re f EMC /Artifact <>BDC 0 .68 .9 0 k 192.615 396.097 1.701 80.047 re f EMC /Artifact <>BDC .043 .133 .827 0 k 198.496 395.406 1.7 19.829 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 200.196 396.097 1.701 52.124 re f EMC /Artifact <>BDC 0 .68 .9 0 k 201.615 396.097 1.701 92.894 re f EMC /Artifact <>BDC .043 .133 .827 0 k 207.496 395.406 1.7 29.557 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 209.196 396.097 1.701 49.357 re f EMC /Artifact <>BDC 0 .68 .9 0 k 210.615 396.097 1.701 84.504 re f EMC /Artifact <>BDC .043 .133 .827 0 k 216.496 395.406 1.7 43.747 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 218.196 396.097 1.701 65.661 re f EMC /Artifact <>BDC 0 .68 .9 0 k 219.615 396.097 1.701 84.504 re f EMC /Artifact <>BDC .043 .133 .827 0 k 225.777 395.346 1.7011 27.729 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 227.478 396.038 1.7009 52.124 re f EMC /Artifact <>BDC 0 .68 .9 0 k 228.897 396.037 1.7011 89.266 re f EMC /Artifact <>BDC .043 .133 .827 0 k 234.777 395.346 1.7011 27.729 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 236.478 396.038 1.7009 62.382 re f EMC /Artifact <>BDC 0 .68 .9 0 k 237.897 396.037 1.7011 87.838 re f EMC /Artifact <>BDC .043 .133 .827 0 k 243.777 395.346 1.7011 20.681 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 245.478 396.038 1.7009 59.144 re f EMC /Artifact <>BDC 0 .68 .9 0 k 246.897 396.037 1.7011 92.124 re f EMC /Artifact <>BDC .043 .133 .827 0 k 252.777 395.346 1.7011 31.883 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 254.478 396.038 1.7008 57.239 re f EMC /Artifact <>BDC 0 .68 .9 0 k 255.897 396.037 1.7011 87.838 re f EMC /Artifact <>BDC q 1 0 0 1 73.915 723.612 cm 0 0 m 0 -102.722 l S Q EMC q 1 0 0 -1 -1191.276 878.88977 cm /Fm18 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm19 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm20 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm21 Do Q q 1 0 0 -1 -1191.276 878.88977 cm /Fm22 Do Q /Artifact <>BDC q 1 0 0 1 74.213 620.89 cm 0 0 m 203.77 0 l S Q EMC /P <>BDC /GS2 gs BT 7 0 0 7 57.5032 724.5582 Tm 0 0 0 1 k 0 Tc (2.5)Tj ET EMC /P <>BDC BT 7 0 0 7 67.548 724.5582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 63.5302 703.3582 Tm (2)Tj ET EMC /P <>BDC BT 7 0 0 7 67.548 703.3582 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 57.5032 682.1583 Tm (1.5)Tj ET EMC /P <>BDC BT 7 0 0 7 67.548 682.1583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 63.5302 660.9583 Tm (1)Tj ET EMC /P <>BDC BT 7 0 0 7 67.548 660.9583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 57.5032 639.7583 Tm (0.5)Tj ET EMC /P <>BDC BT 7 0 0 7 67.548 639.7583 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 63.5302 618.5583 Tm (0)Tj ET EMC /P <>BDC BT /F4 1 Tf 0 8 -8 0 48.3913 665.7013 Tm [(P)69.8(AG S-N)]TJ ET EMC /P <>BDC BT 8 0 0 8 124.0257 596.0693 Tm [(W)39.8(eeks post insemination)]TJ ET EMC /P <>BDC BT /F3 1 Tf 6.79 0 0 7 78.7636 608.8033 Tm ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 80.6106 608.8033 Tm (5)Tj ET EMC /P <>BDC BT 6.79 0 0 7 84.7229 608.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 92.1104 608.8033 Tm 0 Tc (6)Tj ET EMC /P <>BDC BT 6.79 0 0 7 97.0699 608.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 102.6104 608.8033 Tm 0 Tc (7)Tj ET EMC /P <>BDC BT 6.79 0 0 7 107.8198 608.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 113.3605 608.8033 Tm 0 Tc (8)Tj ET EMC /P <>BDC BT 6.79 0 0 7 117.2229 608.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 124.6104 608.8033 Tm 0 Tc (9)Tj ET EMC /P <>BDC BT 6.79 0 0 7 128.422 608.8033 Tm -.015 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 133.9627 608.8033 Tm (10)Tj ET EMC /P <>BDC BT 6.79 0 0 7 143.1157 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 144.9626 608.8033 Tm -.015 Tc (11)Tj ET EMC /P <>BDC BT 6.79 0 0 7 153.8657 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 155.7127 608.8033 Tm -.015 Tc (12)Tj ET EMC /P <>BDC BT 6.79 0 0 7 164.8657 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 166.7126 608.8033 Tm -.015 Tc (13)Tj ET EMC /P <>BDC BT 6.79 0 0 7 175.8656 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 177.7126 608.8033 Tm -.015 Tc (14)Tj ET EMC /P <>BDC BT 6.79 0 0 7 187.1156 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 188.9626 608.8033 Tm -.015 Tc (15)Tj ET EMC /P <>BDC BT 6.79 0 0 7 198.2574 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 200.1043 608.8033 Tm -.015 Tc (16)Tj ET EMC /P <>BDC BT 6.79 0 0 7 208.0188 608.8033 Tm ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 211.7126 608.8033 Tm (17)Tj ET EMC /P <>BDC BT 6.79 0 0 7 220.6157 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 222.4625 608.8033 Tm -.015 Tc (18)Tj ET EMC /P <>BDC BT 6.79 0 0 7 231.3656 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 233.2125 608.8033 Tm -.015 Tc (19)Tj ET EMC /P <>BDC BT 6.79 0 0 7 242.3657 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 244.2125 608.8033 Tm -.015 Tc (20)Tj ET EMC /P <>BDC BT 6.79 0 0 7 253.3656 608.8033 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 255.2125 608.8033 Tm -.015 Tc (21)Tj ET EMC /Artifact <>BDC /GS1 gs q 1 0 0 1 74.215 683.582 cm 0 0 m 12.923 .316 l 25.538 -12.923 l 38.154 -21.23 l 51.692 -18.769 l 63.384 -16.692 l 76.923 -6.771 l 87.692 -7.384 l 102.461 -.615 l 114.236 1.231 l 128 -2.154 l 138.769 4 l 152.504 5.985 l 164.307 -1.538 l 178.015 8.308 l 190.771 5.231 l 203.692 12 l S Q EMC /Artifact <>BDC 0 .68 .9 0 K q 1 0 0 1 74.215 689.223 cm 0 0 m 12.189 -9.333 l 25.866 -22.133 l 37.866 -29.866 l 51.2 -28.533 l 63.733 -29.066 l 75.968 -19.733 l 89.066 -16 l 101.866 -10.133 l 114.236 -4.8 l 126.992 -3.733 l 140 -3.908 l 152.266 -2.666 l 165.26 .5341 l 179.2 -.8 l 190.933 5.334 l 204.8 1.6 l S Q EMC /Artifact <>BDC 1 0 0 0 K q 1 0 0 1 73.948 673.977 cm 0 0 m 12.456 2.446 l 25.867 -10.62 l 38.4 -17.554 l 50.933 -21.02 l 62.933 -14.174 l 77.067 -8.22 l 89.333 1.646 l 103.164 -.754 l 115.467 5.913 l 127.733 9.921 l 140.267 3.78 l 153.333 10.18 l 166.133 3.246 l 178.667 14.173 l 192 14.173 l 204.8 15.78 l S Q EMC /Artifact <>BDC 0 .68 .9 0 k q 1 0 0 1 70.684 689.365 cm 0 0 m 3.0067 3.0067 l 6.0132 .0001 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 82.684 680.365 cm 0 0 m 3.0067 3.0067 l 6.0132 .0001 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC 1 0 0 0 k q 1 0 0 1 74.415 677.199 cm 0 0 m 2.4089 -4.8181 l -2.41 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 86.415 679.199 cm 0 0 m 2.4089 -4.818 l -2.41 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 99.415 666.199 cm 0 0 m 2.409 -4.818 l -2.41 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 112.415 660.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 125.415 656.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 137.415 663.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 150.415 669.199 cm 0 0 m 2.409 -4.818 l -2.41 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 164.415 678.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 176.415 676.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 188.415 682.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC 0 .68 .9 0 k q 1 0 0 1 96.685 667.365 cm 0 0 m 3.0067 3.0067 l 6.0132 .0001 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 109.685 660.365 cm 0 0 m 3.0067 3.0067 l 6.0134 .0001 l 3.0067 -3.0065 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 122.685 660.365 cm 0 0 m 3.0067 3.0067 l 6.0132 .0001 l 3.0067 -3.0065 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 135.685 661.365 cm 0 0 m 3.0066 3.0066 l 6.0131 0 l 3.0066 -3.0067 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 148.685 669.365 cm 0 0 m 3.0067 3.0067 l 6.0132 0 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 160.685 674.365 cm 0 0 m 3.0067 3.0066 l 6.0132 .0001 l 3.0067 -3.0067 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 173.685 679.365 cm 0 0 m 3.0066 3.0067 l 6.0132 .0001 l 3.0066 -3.0065 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 185.685 684.366 cm 0 0 m 3.0067 3.0066 l 6.0132 .0001 l 3.0067 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 210.685 685.366 cm 0 0 m 3.0067 3.0067 l 6.0134 .0001 l 3.0067 -3.0065 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 223.686 687.366 cm 0 0 m 3.0067 3.0067 l 6.0132 0 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 236.686 689.366 cm 0 0 m 3.0067 3.0066 l 6.0132 .0001 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 250.686 688.366 cm 0 0 m 3.0066 3.0065 l 6.0131 -.0001 l 3.0066 -3.0067 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 262.686 694.366 cm 0 0 m 3.0066 3.0066 l 6.0132 -.0001 l 3.0066 -3.0066 l h f* Q EMC /Artifact <>BDC q 1 0 0 1 275.686 690.366 cm 0 0 m 3.0066 3.0066 l 6.0131 0 l 3.0066 -3.0065 l h f* Q EMC /Artifact <>BDC 1 0 0 0 k q 1 0 0 1 201.415 686.199 cm 0 0 m 2.409 -4.8181 l -2.41 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 214.415 680.199 cm 0 0 m 2.4089 -4.8181 l -2.4101 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 227.415 686.199 cm 0 0 m 2.4089 -4.8181 l -2.4101 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 239.415 680.199 cm 0 0 m 2.4089 -4.8181 l -2.4101 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 253.415 691.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 265.415 691.199 cm 0 0 m 2.4089 -4.818 l -2.4101 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC q 1 0 0 1 278.415 692.199 cm 0 0 m 2.4089 -4.8181 l -2.4101 -4.8181 l 0 0 l f* Q EMC /Artifact <>BDC .78 .02 .98 .09 k q 1 0 0 1 77.126 683.88 cm 0 0 m 0 -1.1735 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.252 -1.1735 -4.252 0 c -4.252 1.1736 -3.2995 2.126 -2.126 2.126 c -.9524 2.126 0 1.1736 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 89.126 684.84 cm 0 0 m 0 -1.1735 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.252 -1.1735 -4.252 0 c -4.252 1.1735 -3.2995 2.126 -2.126 2.126 c -.9524 2.126 0 1.1735 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 102.086 670.8 cm 0 0 m 0 -1.1736 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.2519 -1.1736 -4.2519 0 c -4.2519 1.1735 -3.2995 2.126 -2.126 2.126 c -.9524 2.126 0 1.1735 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 114.086 663.84 cm 0 0 m 0 -1.1735 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.2519 -1.1735 -4.2519 0 c -4.2519 1.1735 -3.2995 2.126 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241.166 682.8 cm 0 0 m 0 -1.1736 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.2519 -1.1736 -4.2519 0 c -4.2519 1.1735 -3.2995 2.126 -2.126 2.126 c -.9524 2.126 0 1.1735 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 254.126 691.92 cm 0 0 m 0 -1.1736 -.9524 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.252 -1.1736 -4.252 0 c -4.252 1.1735 -3.2995 2.126 -2.126 2.126 c -.9524 2.126 0 1.1735 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 267.206 689.88 cm 0 0 m 0 -1.1735 -.9525 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.252 -1.1735 -4.252 0 c -4.252 1.1736 -3.2995 2.126 -2.126 2.126 c -.9525 2.126 0 1.1736 0 0 c h f* Q EMC /Artifact <>BDC q 1 0 0 1 280.166 695.88 cm 0 0 m 0 -1.1735 -.9525 -2.126 -2.126 -2.126 c -3.2995 -2.126 -4.252 -1.1735 -4.252 0 c -4.252 1.1736 -3.2995 2.126 -2.126 2.126 c -.9525 2.126 0 1.1736 0 0 c h f* Q EMC /Artifact <>BDC .78 .02 .98 .09 K .5 w q 1 0 0 1 83.112 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 94.255 620.98 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 104.398 620.98 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 115.541 620.981 cm 0 0 m 0 -3.5659 l S Q EMC /Artifact <>BDC q 1 0 0 1 126.684 620.981 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 137.826 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 148.969 620.981 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 160.112 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 171.255 620.98 cm 0 0 m 0 -3.5648 l S Q EMC /Artifact <>BDC q 1 0 0 1 182.398 620.98 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 193.541 620.981 cm 0 0 m 0 -3.5659 l S Q EMC /Artifact <>BDC q 1 0 0 1 204.684 620.981 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 215.826 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 226.969 620.981 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 238.112 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC q 1 0 0 1 247.969 620.981 cm 0 0 m 0 -3.5649 l S Q EMC /Artifact <>BDC q 1 0 0 1 259.112 620.981 cm 0 0 m 0 -3.5651 l S Q EMC /Artifact <>BDC 0 0 0 0 k 249.899 629.668 28.499 34.201 re f EMC /P <>BDC /GS2 gs BT 7 0 0 7 266.9068 658.7311 Tm 0 0 0 1 k 0 Tc (1)Tj ET EMC /P <>BDC BT 7 0 0 7 270.9248 658.7311 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 266.9068 648.3311 Tm (2)Tj ET EMC /P <>BDC BT 7 0 0 7 270.9248 648.3311 Tm ( )Tj ET EMC /P <>BDC BT 7 0 0 7 266.9068 637.9311 Tm (3)Tj ET EMC /Artifact <>BDC 1 0 0 0 k /GS1 gs q 1 0 0 1 258.415 642.199 cm 0 0 m 2.409 -4.818 l -2.41 -4.818 l 0 0 l f* Q EMC /Artifact <>BDC 0 .68 .9 0 k q 1 0 0 1 255.014 650.411 cm 0 0 m 3.4423 3.5195 l 6.9617 .0774 l 3.5194 -3.4421 l h f* Q EMC /Artifact <>BDC 0 0 0 0 k 167.409 484.668 106.583 34.201 re f EMC /P <>BDC /GS2 gs BT 6.79 0 0 7 184.4152 513.7311 Tm 0 0 0 1 k -.015 Tc [(Nadir P)69.7(A)0(G)]TJ ET EMC /P <>BDC BT 6.79 0 0 7 214.5102 513.7311 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 184.4152 503.3311 Tm -.015 Tc [(Initial P)69.7(AG peak)]TJ ET EMC /P <>BDC BT 6.79 0 0 7 229.5161 503.3311 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 184.4152 492.9311 Tm -.015 Tc [(Post-nadir recover)-11.9(y peak P)69.7(A)0(G)]TJ ET EMC /Artifact <>BDC 0 .27 1 0 k /GS1 gs 173.373 514.239 5.1019 5.102 re f EMC /Artifact <>BDC .78 .02 .98 .09 k 173.373 503.239 5.1019 5.102 re f EMC /Artifact <>BDC 0 .68 .9 0 k 173.373 493.239 5.1019 5.102 re f EMC /Artifact <>BDC 0 0 0 0 k /GS5 gs 477.907 400.096 60.647 53.427 re f EMC /P <>BDC /GS2 gs BT 6.79 0 0 7 494.9131 445.3846 Tm 0 0 0 1 k (Low BW)Tj ET EMC /P <>BDC BT 6.79 0 0 7 518.8885 445.3846 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 494.9131 434.9846 Tm -.015 Tc (Medium BW)Tj ET EMC /P <>BDC BT 6.79 0 0 7 531.484 434.9846 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 494.9131 424.5846 Tm -.015 Tc (High BW)Tj ET EMC /P <>BDC BT 6.79 0 0 7 520.749 424.5846 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 494.9131 414.1846 Tm -.015 Tc (Female)Tj ET EMC /P <>BDC BT 6.79 0 0 7 515.5343 414.1846 Tm 0 Tc ( )Tj ET EMC /P <>BDC BT 6.79 0 0 7 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